Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. 00. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. 100 fmol 1536-well (8 µL) 0. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. We would like to show you a description here but the site won’t allow us. Instrument settings can. SpectraMax® Microplate Reader Suite of Products Knowledge Base. tools to expand the boundaries of their research. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. For biochemical assays, the washer removes unbound material and unreacted reagents. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. • Remove media from each well using a blue pipette tip being careful as not to remove cells. The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. Ultra-cooled Photomultiplier Tube and : Silicon photodiode. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. The rate of molecular motion of Protein G slows down when it is bound to antibodies, resulting in an increase of the FP value (Figure 2). Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. 0. A. Consequently, FLIPR has been used. M5. 96-, 384-, and 1536-well microplates. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. Z. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. Microplates, cuvettes (via adapter) Detectors. Molecular Devices SpectraMax Plus Microplate Reader Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader Sale Price: $6,495. #3693; n = 4 for each standard). None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Place the microplate in the drawer. No tools required to assemble. 2. The clear bottom plates offer the advantage of being able to examine the cells by microscopy during the course of the. 96- & 384-well automated washer, multiwash™+ automated washer, 96- and 384-well plates, washer maintenance kit. The device was able to achieve 3σ limits of detection of ca. 5 ng per well. This SpectraMax Plus comes from a long history of reliable tools built by Molecular Devices. Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. Suggested plates are black walled. 500 USD. Get Quote . Service parts for these models are not being produced and are no longer in stock. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in. This can increase throughput and efficiency, thus saving time and space in the lab. Silica excitation fiber, glass emission fiber. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. D SpectraMax® M2 SpectraMax® M2e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part #0112-0102 Rev. 984. SoftMax Pro GxP Software version 5. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. 96- and 384-well. Temperature control range from Ambient +4º to 50ºC. 993 (Figure 3), validating the assay’s usefulness over a wide range of viable cell numbers. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. #C1500) Control standard endotoxin (ACC cat. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. EMax. Expand Microplates and select the newly created plate. 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. Lucid Scientific. The LysoTracker fluorescence at ex/em 574/594 was measured on a Spectramax i3x plate reader (Molecular Devices). sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. 6-384 (96- and 384-well with pipetting) 96-well microplates. Published Dec 16, 2020 | Updated Jun 22, 2023. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. 46Click the Read button in the SoftMax Pro3 reader combines Molecular Devices SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact. 0. TNF standard curve. Learn more. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384. This reader's dual monochromators for. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. EMax. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. C. UV-Star ® 96W Microplate, well working volume 25-370 μL; total well volume 392 μL. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Measurements for every well are automatically tracked over time. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax ® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. 000 to 2. Supersedes 1. Loading Buffer was also added to a set of cell-free wells for background controls. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. More Product Information. Note: if all wells of a 384-well plate are to be read, the kinetic interval may need to be. An enzyme-linked polyclonal antibody specific for mouse/rat IL-22 was added to the wells and. Microplate Spectrophotometers. 005 OD Normal and Rapid modes, 96- and 384-well plates;. 96- and 384-well. This study investigated the efficacy of fermented seaweed (Eucheuma cottonii) on the remediation of fluoride-contaminated soil. Dear Nelly, In a micro plate the fluorescence emission optics are very different from those in a single cell because of obstruction, reabsorption etc. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). x. % transmittance of plates used in a plate reader. com Molecular Devices (Germany) GmbH ismarcring ierach an der iss erman ©2019 Molecular Devices, LLC. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Before dispensing reagents to the microplate, the VersaMax Reader was powered on and SoftMax Pro GxP. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Molecular Devices (UK) Ltd. The EMax Endpoint ELISA Microplate Reader from Molecular Devices (one of the pioneers of microplate detection) provides a simple and durable dedicated endpoint detection method. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Molecular Devices. No accessories are needed and SoftMax® Pro Software. 190. High sensitivity and speed are required for these plate formats if you wish to examine quick kinetic measurements such as calcium fluxes or applications with small signal. For the name of the nearest authorized Molecular Devices Corporation service facility please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. 000 to 2. 96-well) while others have flexibility to. the Bio-Tek PowerWave™ 200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. The Versamax Microplate Reader is built around a grating monochromator. 96- or 384-well microplate compatibility. Thermomax model provides thermal control and vertical shake, perfect for. robotics-automation-compatible. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Plate formats 6, 12, 24, 48, 96, 384 wells Light source Xenon flash lamp (1 joule/flash) Detector Photomultiplier (R-3896). The equation that allows one to calculate absorbance from % transmittance is. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. The VICTOR Nivo. * Note: Programming the washer with a final aspirate height of 4. Page 1 of 8 . x. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Refurbished. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. com. Other. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. We have the following plate-reader instrument available for on-site screens and other projects Molecular Devices Spectramax Paradigm for luminescence, fluorescence, and. Optional 12-way manifold) 5032336. An industry-renowned instrument for monitoring GPCRs and ion channels. Published Dec 16, 2020 | Updated Nov 07, 2022. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). MCF7 cells treated with. 96/384-well plate formats. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate: 0517 Lit. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Learn how you can utilize microplate readers to drive landmark discoveries. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. This page contains the. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. Important things to consider when purchasing a multimode microplate reader. Or scan up to 384 wells at any time to determine the best assay conditions. Bay Gray powder coated metal back plate, column, and base. Product Compare 0. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. #E010) Pyroplate 96-well microplate (ACC cat. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Or scan up to 384 wells at any time to determine the best assay conditions. External Dimensions without lid (mm). EIGHT-CHANNEL OPTICS Plate reads as fast as five seconds MICROPLATES OR CUVETTES Use a 96- or 384-well microplate or a standard cuvette The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. This can increase throughput and efficiency, thus saving time and space in the lab. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. 039. Cell viability and cytoxicity assays. The IC50s by cell rounding and LysoTracker. We would like to show you a description here but the site won’t allow us. Materials. QuickSync. REF (SpectraMax. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. On the day of the assay, the plate was equilibrated. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Each plate reader or washer is calibrated, inspected, and lubricated—any potential issues . x. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. Three kits covering a wide range of dsDNA concentrations. The soil was amended with either 1. This is a Molecular Devices Thermomax 96 well Absorbance Microplate Readers. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). What is the dead volume in 96 and 384 well compound plates? Dead volume is 5 micro liters. Figure 3. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. CO 2 & O 2 control. • Easy and gentle immobilization of biotin. Compare Tool. The procedures in this protocol support the 96-well reaction plate. Absorbance equation. ~ Bio-Tek ELx808 Microplate Reader $2,795. The drawing shows a standard microplate of 15 mm height covered with a lid. Microplate Reader Ordering information Item Description Part number FLIPR Membrane. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. Polypropylene deep well plates, round bottom. Features. Microplate readers, SpectraMax®, with SpectraDrop. Figure 3. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. 1. $488. Absorbance Plate Reader Comparison Chart. S. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. style. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. SpectraDrop High-Throughput Screening (HTS) Kit. Download a PDF version of the SpectraTest validation package User Guide and the ABS2 SpectraTest validation protocols. Primary considerations when choosing a microplate reader are throughput and flexibility. 96-well plate: 0 to 4 Abs, ±2% at 450 nm, 384-well plate: 0 to 3 Abs ±2% at 450 nm: Measurement Type: Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra: No. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. 96-, 384-, and 1536-well microplates. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. 3. 000 ABS • Resolution: 0. and glow luminescence modes in 96- to 384-well plates. Three low volume 24-well bottom slides. Microplate readers from Tecan; cutting edge bench-top microplate readers lead the industry in both versatility and performance. It features a modular design that allows users to add or change detection cartridges according to their needs. 005 OD Normal and Rapid modes, 96- and 384-well plates Fluorescence Read Timing Excitation Filter: 485/20 nm Emission Filter: 528/20 nm 96-well read: 89 sec. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. Our. UV absorbance detection. 96- and 384-well microplates. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Never order or replace filters to optimize an assay again!6-, 12-, 24-, 48-, 96- and 384-well plates: Tablet PC: Windows® 8. Use standard plate unless you see your type listed. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well…. Features. A barcode reader accessory can be purchased with theWe would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Align the two slots on the bottle holder with the two pegs on the rail. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Here, we demonstrate validation of the SpectraMax ® i3x Multi-Mode Microplate Reader for NanoBRET assays. 030 fmol Fluorescence Polarization (FP). ©2022 Molecular Devices - All rights. 100 ˜L (96-well) or 25 ˜L (384-well) of Loading Buffer was added to each assay well. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. 000 – 4. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. After incubation, the assay plate is transferred to the seal-peeler to remove the seal from the assay plate and transferred to washing. Plate Storage Solutions for Liquid Handling Automation Remote Lab Management. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. x. Plate. Up to 96 well plates, there is often a good signal that can easily be detected, and 96 wells are measured comparatively quickly. Tristar 3 Multimode Microplate Reader. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. adjust the reader. The CellTiter-Glo assay was used to measure ATP. B. Published Dec 16, 2020 | Updated Dec 16, 2020. 003 fmol 0. This 96-well assay has been fully validated on the SpectraMax iD5 reader and other Molecular Devices microplate readers with FP detection to ensure reliable results. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. The color touch screen provides a visual user interface, making programming fast and intuitive. With various on-board features and capabilities, the ThermoMax. M2. Meanwhile, Cowan discusses the importance of considering plate well densities and the angle from which microplates are read inside the reader: “You should definitely check the range of plate well densities supported by your candidate readers. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. 5% at 1. 1. Therefore you need to ask a physicist. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. MultiWash+ for 96-well plates: 4 liquid lines, 8-way manifold and tubing, 4 wash/rinse bottles (2L), 1 waste bottle (2. 96-well plates - Filter plates with 0. ₹ 1,80,000 Get Latest Price. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. g. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. In SoftMax Pro, Dual Read mode was selected. 3) At the bottom of the strip, click on "Show. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. and California)The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. #iD5-STD). D. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Molecular Devices, LLC Plate Formats: 96 or 384 Well Plates; Manifold Channels: Inquire; Dispensing Liquids: Inquire; Citations: Get Quote. Yes—whole plate dispenser (96 or 384 well) Hamamatsu: FDSS7000EX: High throughput screening system: Yes (optional feature). Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. The SpectraMax ABS/ABS Plus Microplate Readers provide fast absorbance detection without the use of filters with monochromator-based wavelength selection for visible and. Features of Streptavidin Coated Plates include: • Approximate binding capacity is ∼5 pmol biotin/well. 384-WELL SBS STERILE MICROPLATE. Instrument settings can beIntegrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. Well scans from the Gemini EM Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM/Ethidium Homodimer-1) assay kit. 303 unable to cal dark current. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. #E010) Pyroplate 96-well microplate (ACC cat. the Gemini XPS reader. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. We would like to show you a description here but the site won’t allow us. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Molecular Devices LLC Applications: Inquire; Plate Formats: 96-, 384- well. UV absorbance detection. 0 . The patented quench technology is offered to drug discovery and life science researchers exclusively by Molecular Devices, through the purchase of FLIPR Assay Kits. x. Wells to read. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. 1 mL/well of media. Both 96- and 384-well assay formats yielded similar linearity and dynamic range, indicating the suitability of the dual-luciferase assay and SpectraMax i3x system for both formats. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. . During preincubation, the working reagent was prepared by mixing fluorogenic substrate, assay buffer, and rFC enzyme solution in a 5:4:1 ratio, respectively. 20 µL (96-well plates) or 10 µL (384. Published Dec 16, 2020 | Updated Dec 06, 2021. Recertification service. minutes and begin reading microplates with seven mouse clicks. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Make sure toPlate reader instructions Computer. Please click on the links below to view the Microplate Reader Comparison Tables. 00 . 1. Products : Ionworks HT, Ionworks Quattro. 0200-6262. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. Some instruments can handle only 96-well plates and lower, whereas others can read up to the ultrahigh throughput densities of 1,536-well plates. $3,207. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads, the VMax Microplate Reader has both speed and performance. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. Plus 384. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. • 0. The. 4: Berthold Technologies: TriStar LB 941: Multimodal plate reader: Yes:. LogPhase 600 is controlled with a software App that provides a simple, yet powerful, user experience. These plates are compatible with standard fluorescence microplate readers and are offered in a. Two days before the experiments, cells are seeded into the clear-bottom 96-well plates (approximately 10,000 cells per well for cancer cell lines and 30,000–40,000 cells per well for fibroblasts and NPC; see Note 5) and cultured in the media suited for users’ cell lines. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Microplates, cuvettes (via adapter) Detectors. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. The Best Microplate Readers According to our Product Reviewers (1) Write a Review. Automated live cell imaging – cell counting and confluence. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. 4ti-0255 96 well assay plate, pyramid flat bottom, clear, non-sterile UV wavelength 4ti-0214 UltraVision Plate 384, black, non -sterile, with UV baseELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. S. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. FAST AND ACCURATE METERTECH Microplate Reader $3,650. Each wellThe Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. #ABS Plus) Cell plating and treatment prior to assay. Selects either 96-well or 384-well plate for a stand-alone read. variety of. Microplate Readers include the M3, M4, M5, and M5e models. 2. The plate reader can measure multiple readouts, including UV and. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Contents of wells were pipetted up and down several times to mix. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. It offers extremely low absorbance background, excellent. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Bioz Stars score: 86/100, based on 40 PubMed citations. The picked. Answer. d. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Plate Shaking. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Accurate, efficient nucleic acid quantitation is critical for molecular biology labs that work with precious, rare, or difficult-to-process samples and applications requiring precise measurement. Visit the supplier page or submit a quote to learn more.